Translational_Unit

Part:BBa_K1500004:Design

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


IPTG-GFP MutD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1508
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

None


Source

PCR'd from DH5-alpha genomic sequence, with RBS.3 (medium) added as an overhang in front of the gene.

References